HSV-1 rGlycoprotein D
| Virus Strain | MacIntyre |
| Host Cell | Baculovirus (Sf-9) |
| Cell Line Characterization | Mycoplasma negative |
| Purity | Infected cell extract enriched for viral proteins |
| Inactivation | Greater than 90% by SDS-PAGE |
| Supplied In | Phosphate Buffered Saline pH 7.4 |
| Protein Concentration | 1.0 mg/ml |
| Fill Volume | 103 µl (H1V132-100) or 1.03 ml (H1V132-1) |
| ELISA (IgG) Titer | >1:64,000 |
| Western Blot Titer | 10 µg/cm |
| Reference and/or Additional Reading | J. Virology (1984), 49(1):102 |
ELISA Results
Antigen is Virusys # H1V016 HSV-1 Infected Cell Extract AND H1V132 HSV Recombinant gD-1 coated at various dilutions in PBS. Primary antibodies are human plasmas previously characterized for HSV-1 IgG serology. See SOP-138 for procedure.
SDS-PAGE and Western Blot Results
Antigen is Virusys # H1V132 HSV-1 Recombinant gD at 10 µg/cm. Primary antibody is HA025-100 HSV gD Monoclonal Antibody. See SOP-128 for procedure.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.
