HSV-1 rGlycoprotein G
| Virus Strain | MacIntyre |
| Host Cell | Baculovirus (Sf-9) |
| Cell Line Characterization | Mycoplasma negative |
| Purity | Infected cell extract enriched for viral proteins |
| Inactivation | Greater than 90% by SDS-PAGE |
| Supplied In | Phosphate Buffered Saline, 6M Urea pH 7.4 |
| Protein Concentration | 2.2 mg/ml |
| Fill Volume | 45 µl (H1V133-100) or 450 µl (H1V133-1) |
| ELISA (IgG) Titer | >1:64,000 |
| Western Blot Titer | 10 µg/cm |
| Reference and/or Additional Reading | J. Virol. Methods (1986), 14:111 |
ELISA Results
Antigen is Virusys # H1V016 HSV-1 Infected Cell Extract AND H1V133 Recombinant HSV gG-1 coated at various dilutions in PBS. Primary antibodies are human plasmas previously characterized for HSV-1 IgG serology. See SOP-138 for procedure.
SDS-Page and Western Blot Results
Antigen is Virusys # H1V133 HSV-1 Recombinant gG at 10 µg/cm. Primary antibody is H1A020-100 HSV-1 gG Monoclonal Antibody. See SOP-128 for procedure.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.
