Appropriate Control For:

Host Cell

Cell Line Characterization

Purity

Inactivation

Supplied in

Protein Concentration

Fill Volume

ELISA (IgG) Titer

Western Blot Titer

Reference and/or Additional Reading

Baculovirus/Sf9 infected cell extracts 

Spodoptera frugiperda (Sf9)

Negative for mycoplasma and bacteria

Uninfected cell extract

None

Phosphate Buffered Saline pH 7.4  

0.98 mg/ml 

1.03 ml (AG141-1) or 25.2 ml (AG141-25) 

N/A

N/A 

In Vitro 13: 213-217 (1977)

ELISA Results

Antigens are # AG141 Sf9 Uninfected Cell Extract  and 2 different 6-his tagged protein extracts coated at various dilutions in PBS. Primary antibody is chicken anti-6-his at 1:1,000.

See SOP-138 for procedure.
 

SDS-PAGE Results

Antigen is Virusys # AG141 Uninfected Sf9 Cell Extract at 10 µg/cm. See SOP-128 for procedure.

This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C.  Product degradation will result from multiple freeze/thaw cycles.  It is suggested that the antigen be stored in use size aliquots and thawed just prior to use.  Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.