E. coli Expression Optimization
Our experience has been that recombinant protein expression is dramatically affected by variables such as fermentation broth, carbon source, IPTG concentration and cell density at induction. We will systematically investigate these variables for your transformed bacteria. When you move to higher volume fermentations, you will know that you are using the best conditions possible in the production of your protein.
One study of 5 broths, 3 carbon sources, 3 induction conditions
2 - 3 weeks
A summary of best fermentation broth and induction conditions.