Question:

What is the recommended dilution for the use of this antibody in a Western Blot?

Will this antibody recognize recombinate gG1 and native HSV-1 virus in a western blot?

Resolution:

This antibody was tested on HSV-1 MacIntyre extract at 10 µg/cm lane width.

Antibody concentration was 10 µg/ml (1:100) and incubation was 60 mintes 37°C. Detection was AP/BCIP/NBT.

The antibody definitely recognizes native gG-1. It will also recognize recombinant gG-1, depending on your construct.