Bovine Serum Albumin (BSA) Antigen Capture ELISA - Photometric - 5 plate kit

The advent of biological products produced using animal components has created a need to ensure their removal prior to use. This removal can be accomplished in a number of ways but in all cases, must be verified by the use of a sensitive and specific assay methodology. Fetal calf serum is commonly used in tissue culture processes in concentrations of 1 – 10%. Guidelines published by a 1987 WHO study group (WHO Tech. Rep. Ser., 747, 1987) suggest a residual concentration of no more than 1 part per million in the final product (1 µg/ml). Since fetal calf serum is largely Bovine Serum Albumin, this protein was chosen as the basis of the current assay. The assay can be completed in less than 2 hr. The test kit incorporates proprietary diluents that are designed to prevent the development of nonspecific signal derived from complex sample matrix effects and/or the nonspecific adsorption of reactive test components, resulting in improvements in both sensitivity and specificity. Two- and five-plate test kits are available in a standard photometric detection format (AK249-2 or AK249-5).