Target Protein |
Cytomegalovirus pp65 Lower Matrix Protein |
Target Protein Also Known As |
UL83, LMP, vp65 |
Clone Designation |
3A12 |
Species/Isotype |
Mouse IgG2A(k) |
Purity |
Affinity Purified, > 95% IgG by SDS-PAGE |
Supplied in |
Phosphate Buffered Saline pH 7.4 (no azide) |
Protein Concentration |
1.0 mg/ml |
Fill Volume |
103 µl (100 µg) or 1030 µl (1 mg) |
Immunofluorescence Titer |
1+ @ 1:3,200 |
ELISA Titer |
0.100 @ 1:102,400 |
Western Blot Titer |
> 1:16,000 |
Reference / Additional Reading |
J. Virology (1987), 61(2):446 |
Immunofluorescence Results
Procedure uses Virusys # CG015 10-well CMV slides, # AG002 anti-Mouse-FITC F(ab')2 w/ counterstain and # AG065 StayBright Mounting Media.
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Cross Reactivity Results
Determined by IFA at an antibody dilution of 1:100 with Virusys # AG002 anti-Mouse-FITC F(ab')2 w/ counterstain and # AG065 StayBright Mounting Media.
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ELISA Results
Antigen is Virusys # CV001 CMV Infected Cell Extract coated at a dilution of 1:100.
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Western Blot Results
Antigen is Virusys # CV001 CMV Infected Cell Extract at 10 µg/cm.
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ImmunoHistoChemistry (IHC)
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This product is intended for research and manufacturing uses only. It is not a diagnostic device. Upon receipt, store at -20°C or below. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antibody be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to two weeks is acceptable for unused portions.