Cytomegalovirus Real Time PCR
Virusys Corporation’s CMV qPCR 20x probe mix is a reliable probe for measuring relative amounts or total copy numbers of CMV by quantitative PCR. The probe consists of forward and reverse primers combined with a FAM/BHQ-labeled probe specific for the UL83 region of CMV. This dual-labeled probe is supplied with PCR primers at 20x concentration and may be used with standard real-time PCR reagents.
For absolute copy number calculations, a tube of standards is also included containing the target sequence at 1012 copies per ml. These standards produce linear results at dilutions of 108 to 102 copies per reaction.
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