EBV Negative Control Extract Lot I1857120
| Catalog Number | EV139-1, EV139-25 |
| Appropriate Control for | EBV |
| Host Cell | Human B Cell |
| Cell Line Characterization | Confirmed negative for HIV and Hepatitis B |
| Purity | Uninfected cell extract mock enriched for viral proteins |
| Inactivation | Inactivated as if infected - use as control for EV012 EBV Infected Cell Extracts |
| Supplied In | 100 mM Glycine pH 9.5 |
| Protein Concentration | 0.97 mg/ml |
| Fill Volume | 1.03 ml (EV139-1) or 25.2 ml (EV139-25) |
| ELISA (IgG) Titer | None |
| Western Blot Titer | 50 µg/cm |
| Reference and/or Additional Reading | None - proprietary human B-cell line |
ELISA Results
Antigen is Virusys # EV012 EBV Infected Cell extract AND # EV139 EBV Negative cell extract coated at various dilutions in PBS. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-138 for procedure.
Western Blot Results
Antigen is Virusys # EV139 EBV Negative cell extract at 50 µg/cm. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-128 for procedure and see EV012 EBV Infected Cell extract for banding patterns of sera.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.
