EBV Negative Control Extract Lot I1857120
|Appropriate Control for
|Human B Cell
|Cell Line Characterization
|Confirmed negative for HIV and Hepatitis B
|Uninfected cell extract mock enriched for viral proteins
|Inactivated as if infected - use as control for EV012 EBV Infected Cell Extracts
|100 mM Glycine pH 9.5
|1.03 ml (EV139-1) or 25.2 ml (EV139-25)
|ELISA (IgG) Titer
|Western Blot Titer
|Reference and/or Additional Reading
|None - proprietary human B-cell line
Antigen is Virusys # EV012 EBV Infected Cell extract AND # EV139 EBV Negative cell extract coated at various dilutions in PBS. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-138 for procedure.
Western Blot Results
Antigen is Virusys # EV139 EBV Negative cell extract at 50 µg/cm. Primary antibodies (RN09 - AN1) are human plasmas previously characterized for EBV serology. See SOP-128 for procedure and see EV012 EBV Infected Cell extract for banding patterns of sera.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.