Virusys Corporation’s HHV-8 qPCR 20x probe mix is a reliable probe for measuring relative amounts or total copy numbers of HHV-8 by quantitative PCR. The probe consists of forward and reverse primers combined with a FAM/BHQ-labeled probe specific for ORF26 (capsid triplex protein) of HHV-8. This dual-labeled probe is supplied with PCR primers at 20x concentration and may be used with standard real-time PCR reagents.

For absolute copy number calculations, a tube of standards is also included containing the target sequence at 10¹² copies per ml. These standards produce linear results at dilutions of 10⁸ to 10² copies per reaction.

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H8K245 HHV8 PCR.pdf