HSV-2 rGlycoprotein G
| Virus Strain | "G" |
| Host Cell | Pichia Pastoris |
| Cell Line Characterization | Mycoplasma negative |
| Purity | Infected cell extract enriched for viral proteins |
| Inactivation | Greater than 90% by SDS-PAGE |
| Supplied In | 50 mM Tris, 5 mM DTT, 1 mM EDTA, 0.1% Triton X-100 pH 8 |
| Protein Concentration | 0.4 mg/ml |
| Fill Volume | 232 µl (H2V220-100) or 1.0 ml (H2V220-1) |
| ELISA (IgG) Titer | >1:64,000 |
| Western Blot Titer | 10 µg/cm |
| Reference and/or Additional Reading | J. Virol. Methods (1992), 36:249 |
ELISA Results
Antigen is Virusys # H2V017 HSV-2 Infected Cell Extract AND H2V220 HSV Recombinant gG-2 coated at various dilutions in PBS. Primary antibodies are human plasmas previously characterized for HSV-2 IgG serology. See SOP-138 for procedure.
SDS-PAGE and Western Blot Results
Antigen is Virusys # H2V220 HSV-2 Recombinant gG at 10 µg/cm. Primary antibody is H2A023-100 HSV gG Monoclonal Antibody. See SOP-128 for procedure.
| SDS-PAGE | Western |
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This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.
