Thrombin Cleavage of Fusion Protein Tag
Your protein is cleaved from it's purification tag using the thrombin cleavage site that is often included at the junction of the tag and your protein. Since every protein cleaves with slightly different kinetics, a time course and thrombin titration is performed to determine the optimum conditions for your protein. After digestion, the tag and residual thrombin are purified away to leave a purified "non-fused" recombinant protein.
1 - 2 weeks
Highly variable depending on individual protein characteristics.
A Certificate of Analysis is provided with your purified protein that provides the following data:
To obtain the final western blot data, you must supply us with an appropriate antibody as without the tag our anti-tag antibody will no longer work to detect your protein.