Question:
I am looking for a preparation that I can use as a positive control for RT-PCR of CMV proteins. Are the cell extracts you sell depleted of RNAs or would they be useful for RT-PCR?
Resolution:
The infected cell extracts are not your best source of nucleic acid as they are processed for protein (including a protein specific precipitation step) and extensively sonicated. If you are after mRNA, then the purified virus preparations would not work either.
If you like, we can quote you on a mRNA extraction of an active infection.