Question:

Are the HSV1 and 2, CMV, EBV antigens good for both IgG and IgM detection?

Resolution:

All of the antigens, including the EBV extract, are good for IgG.

The purified EBV-VCA is intended for EBV-IgM.

Any of the extracts can work for IgM with the understanding that background will be a higher and positive/negative discrimination lower. IgM assays generally require more optimization than IgG and therefore are more difficult to make. There are ways to enhance certain antigens for IgM detection but these must be addressed in an individual fashion.