Question:

Can you strip westerns so that they can be used with another set of antibodies?

Resolution:

Not yet evaluated, so use with care.

  1. Put the membrane in PBS or TBS with 7ul/ml B-mercaptoetanol and 2%SDS for 30' at RT in agitation.
  2. Wash the membrane for 30' in PBS or TBS.
  3. Put the membrane in blocking buffer.