To describe the formulation of 10X Tris-Glycine-SDS electrophoresis running buffer.


Polyacrylamide gel electrophoresis in SDS containing buffers using multiphasic buffer systems: Properties of the stack, valid Rf measurement and optimized procedure. Anal. Biochem. (1978), 78:459


  • RMS-110 Tris
  • RMS-123 Glycine
  • RMS-177 Sodium Dodecyl Sulfate (SDS)


  • Stir Plate and stir bar
  • Glassware
  • pH meter and pH standards



  1. Add the following to 880 ml DI water:
         - 30.3 g Tris
         - 144.1 g Glycine
         - 10 g SDS
  2. Stir to dissolve.
  3. Standardize pH meter to pH 7 and 10.
  4. Check pH of solution. It should be between 8.6 and 8.9.  DO NOT ADD ACID OR BASE TO ADJUST pH.
  5. Label with contents, lot number and one year expiration date.