VZV Purified Glycoproteins Lot H1861007
| Virus Strain | Ellen |
| Host Cell | Normal Human Dermal Fibroblast (NHDF) |
| Cell Line Characterization | Confirmed Negative for HIV and Hepatitis B |
| Purity | Lectin affinity purified |
| Inactivation | Verified by tissue culture infectivity assay. |
| Supplied In | 50 mM tris, 1M NaCl, 1 mM MgCl2, 0.1% triton X-100, 0.2M Methylmanno pyrannoside pH 7.5 |
| Protein Concentration | 0.40 mg/ml |
| Fill Volume | 1.03 ml (VV147-1) or 25.2 ml (VV147-25) |
| ELISA (IgG) Titer | >1:1,600 |
| Western Blot Titer | 10 µg/cm |
| Reference and/or Additional Reading | J. Med. Virology (1990), 32:189 |
ELISA Results
Antigen is Virusys # VV147 VZV Purified Glycoproteins coated at various dilutions in PBS. Primary antibodies are human plasmas previously characterized for VZV serology. See SOP-138 for procedure.
Western Blot Results
Antigen is Virusys # VV147 VZV Purified Glycoproteins at 10 µg/cm. Primary antibodies are human plasmas previously characterized for VZV serology. See SOP-128 for procedure.
This product is intended for research and manufacturing uses only. It is not a diagnostic device. It is supplied frozen on dry ice - upon receipt, store at -70°C. Product degradation will result from multiple freeze/thaw cycles. It is suggested that the antigen be stored in use size aliquots and thawed just prior to use. Storage at 2 - 8°C for up to twelve hours is acceptable for unused portions.
