One goat is immunized via intramuscular injection with client supplied antigen. Towards the end of the protocol, 3 production bleeds are taken and the animals placed on rest pending your testing results.
1 animal immunized 5 times. Three production bleeds are pooled together for a total of approximately 1,000 ml serum at project completion.
Approximately 14 weeks
Production bleeds are approximate 300 ml serum per animal. Exanguination bleeds are approximately 1000 ml per animal. Purified antibody yield (total) can be expected to be approximately 20 - 30 mg/ml serum while specific antibody can be approximately 0.1- 0.5 mg/ml depending on the characteristics of the immunizing antigen..
Documentation regarding animal and immunogen.
Client needs to provide 2 milligrams antigen at a minimum of 0.5 mg/ml in PBS .